Transcriptome analysis identifies genes involved in adventitious branches formation of Gracilaria lichenoides in vitro

• DOI: 10.1038/srep17099
• Publication Date: 2015-12-11T10:18:13Z
• Authors: Wenlei Wang; Huanqin Li; Xiangzhi Lin; Shanjun Yang; Zhaokai Wang; Baishan Fang
• Keywords: Callus

Abstract Tissue culture could solve the problems associated with Gracilaria cultivation, including consistent supply of high-quality seed stock, strain improvement and efficient mass high-yielding commercial strains. However, STC lags behind that higher plants because paucity genomic information. Transcriptome analysis identification potential unigenes involved in formation regeneration callus or direct induction ABs are essential. Herein, CK, EWAB NPA G. lichenoides transcriptomes were analyzed using Illumina sequencing platform first time. A total 17,922,453,300 nucleotide clean bases generated assembled into 21,294 unigenes, providing a gene space 400,912,038 nucleotides an average length 1,883 N 50 5,055 G + C content 52.02%. BLAST resulted assignment 13,724 (97.5%), 3,740 (26.6%), 9,934 (70.6%), 10,611 (75.4%), 9,490 (67.4%) 7,773 (55.2%) annotated to NR, NT, Swiss-Prot, KEGG, COG GO databases, respectively was 14,070. 17,099 transcripts predicted possess open reading frames, 3,238 13,861 blasted based on protein databases. In addition, 3,287 SSRs detected G.lichenoides , further support for genetic variation marker-assisted selection future. Our results suggest auxin polar transport, signal transduction, crosstalk other endogenous plant hormones antioxidant systems, play important roles explants vitro . The present findings will facilitate studies discovery molecular mechanisms underlying tissue seaweed.