iTRAQ proteomic analysis of extracellular matrix remodeling in aortic valve disease

• DOI: 10.1038/srep17290
• Publication Date: 2015-12-01T09:54:27Z
• Authors: Tatiana Martin-Rojas; Laura Mourino-Alv...; Sergio Alonso-Orgaz; Esther Rosello-Lleti; Enrique Calvo; Luis Fernando Lop...; Miguel Rivera; Luis R. Padial; Juan Antonio Lopez; Fernando de la Cu...; Maria G. Barderas
• Keywords: Periostin; Proteome

Abstract Degenerative aortic stenosis (AS) is the most common worldwide cause of valve replacement. The a thin, complex, layered connective tissue with compartmentalized extracellular matrix (ECM) produced by specialized cell types, which directs blood flow in one direction through heart. There evidence suggesting remodeling such ECM during development. Thus, better characterization role proteins this disease would increase our understanding underlying molecular mechanisms. Aortic samples were collected from 18 patients underwent replacement (50% males, mean age 74 years) and normal control valves obtained necropsies (40% 69 years). proteome was analyzed 2D-LC MS/MS iTRAQ methodology. results showed an altered expression 13 3 (biglycan, periostin, prolargin) validated Western blotting and/or SRM analyses. These findings are substantiated previous demonstrating differential protein expression. present study has demonstrated pattern individuals AS, therefore supporting dynamic human AS