Mesenchymal stem cell-laden anti-inflammatory hydrogel enhances diabetic wound healing

• DOI: 10.1038/srep18104
• Publication Date: 2015-12-08T10:16:12Z
• Authors: Shixuan Chen; Junbin Shi; Min Zhang; Yinghua Chen; Xueer Wang; Lei Zhang; Zhihui Tian; Yuan Yan; Qinglin Li; Wen Zhong; Malcolm Xing; Lu Zhang; Lin Zhang
• Keywords: Granulation tissue; Cell therapy

Abstract The purpose of this study was to permit bone marrow mesenchymal stem cells (BMSCs) reach their full potential in the treatment chronic wounds. A biocompatible multifunctional crosslinker based temperature sensitive hydrogel developed deliver BMSCs, which improve inflammation microenvironments detailed vitro investigation found that is suitable for BMSC encapsulation and can promote secretion TGF-β1 bFGF. In vivo , full-thickness skin defects were made on backs db/db mice mimic diabetic ulcers. It revealed inhibit pro-inflammatory M1 macrophage expression. After association with BMSCs treated wound, significantly greater wound contraction observed + group. Histology immunohistochemistry results confirmed contributed rapid healing wounds by promoting granulation tissue formation, angiogenesis, extracellular matrix secretion, re-epithelialization. These show a laden may be promising therapeutic strategy management