Profiles of microRNA networks in intestinal epithelial cells in a mouse model of colitis

• DOI: 10.1038/srep18174
• Publication Date: 2015-12-09T10:24:17Z
• Authors: Juneyoung Lee; Eun Jeong Park; Yoshikazu Yuki; Shandar Ahmad; Kenji Mizuguchi; Ken J. Ishii; Motomu Shimaoka; Hiroshi Kiyono
• Keywords: Pathogenesis; Intestinal mucosa; Homeostasis

Abstract Inflammatory bowel diseases (IBDs) accompany a critical loss of the frontline barrier function that is achieved primarily by intestinal epithelial cells (IECs). Although gene-regulation pathways underlying these host-defense roles IECs presumably are deranged during IBD pathogenesis, quantitative and qualitative alterations posttranscriptional regulators such as microRNAs (miRNAs) within largely remain to be defined. We aimed uncover regulatory miRNA–target gene relationships arise differentially in inflamed small- compared with large-IECs. Whereas significantly increased expression only few miRNA candidates small-IECs, numerous miRNAs were upregulated These marked might explain why large, small, intestine more sensitive colitis shows severe pathology this experimental model IBD. Our in-depth assessment miRNA–mRNA profiles resulting networks prompts us suggest miR-1224, miR-3473a miR-5128 represent biomarkers appear large-IECs upon development co-operatively repress key anti-inflammatory factors. The current study provides insight into gene-regulatory through which dynamic rearrangement involved modulates expression–regulation machinery between maintaining disrupting gastrointestinal homeostasis.