Functional Analysis of Esterase TCE2 Gene from Tetranychus cinnabarinus (Boisduval) involved in Acaricide Resistance

• DOI: 10.1038/srep18646
• Publication Date: 2016-01-04T10:11:30Z
• Authors: Li Shi; Peng Wei; Xiangzun Wang; Guangmao Shen; Jiao Zhang; Wei Xiao; Zhifeng Xu; Qiang Xu; Lin He
• Keywords: Abamectin; Heterologous expression; Tetranychus; Esterase; Heterologous

Abstract The carmine spider mite, Tetranychus cinnabarinus is an important pest of crops and vegetables worldwide it has the ability to develop resistance against acaricides rapidly. Our previous study identified esterase gene (designated TCE2 ) over-expressed in resistant mites. To investigate this gene’s function resistance, expression levels susceptible, abamectin-, fenpropathrin- cyflumetofen-resistant strains were knocked down (65.02%, 63.14%, 57.82% 63.99%, respectively) via RNA interference. bioassay data showed that three significantly decreased after down-regulation , indicating a correlation between acaricide-resistance T. . was then re-engineered for heterologous Escherichia coli recombinant exhibited α-naphthyl acetate activity (483.3 ± 71.8 nmol/mg pro. min −1 enzyme could be inhibited by abamectin, fenpropathrin cyflumetofen, respectively. HPLC GC results 10 μg effectively decompose 21.23% 49.70% cyflumetofen within 2 hours. This first report successful from provides direct evidence functional involved acaricide cinnabarinus.