Hypoxia-inducible miR-210 contributes to preeclampsia via targeting thrombospondin type I domain containing 7A

• DOI: 10.1038/srep19588
• Publication Date: 2016-01-22T10:01:25Z
• Authors: Rongcan Luo; Yongqing Wang; Peng Xu; Guangming Cao; Yangyu Zhao; Xuan Shao; Yu-xia Li; Cheng Chang; Chun Peng; Yan-ling Wang
• Keywords: Hypoxia

Abstract Preeclampsia, a relatively common pregnancy disorder, is major contributor to maternal mortality and morbidity worldwide. An elevation in microRNA-210 (miR-210) expression the placenta has been reported be associated with preeclampsia. Our bioinformatic analysis showed that thrombospondin type I domain containing 7A (THSD7A) predicted target for miR-210. The aim of this study was determine whether miR-210 involved preeclampsia through its targeting THSD7A human placental trophoblasts. In preeclamptic tissues, levels were significantly downregulated inversely correlated validated as direct using quantitative real time PCR (qRT-PCR), Western blotting dual luciferase assays HTR8/SVneo cells. Transwell insert invasion mediated invasion-inhibitory effect Interestingly, hypoxia markedly increased while suppressing time-dependent manner This provides novel data on function cells extends our knowledge how development