Effects of K-115 (Ripasudil), a novel ROCK inhibitor, on trabecular meshwork and Schlemm’s canal endothelial cells

• DOI: 10.1038/srep19640
• Publication Date: 2016-01-19T09:32:10Z
• Authors: Yoshio Kaneko; Masayuki Ohta; Toshihiro Inoue; Ken Mizuno; Tomoyuki Isobe; Sohei Tanabe; Hidenobu Tanihara
• Keywords: Schlemm's canal; Rho kinase inhibitor

Abstract Ripasudil hydrochloride hydrate (K-115), a specific Rho-associated coiled-coil containing protein kinase (ROCK) inhibitor, was the first ophthalmic solution developed for treatment of glaucoma and ocular hypertension in Japan. Topical administration K-115 decreased intraocular pressure (IOP) increased outflow facility rabbits. This study evaluated effect on monkey trabecular meshwork (TM) cells Schlemm’s canal endothelial (SCE) cells. induced retraction rounding cell bodies as well disruption actin bundles TM In SCE-cell monolayer permeability studies, significantly transendothelial electrical resistance (TEER) flux FITC-dextran. Further, disrupted cellular localization ZO-1 expression monolayers. These results indicate that decreases IOP by increasing association with modulation behavior SCE tight junction.