Immunoglobulin G Expression in Human Sperm and Possible Functional Significance

• DOI: 10.1038/srep20166
• Publication Date: 2016-02-01T10:51:17Z
• Authors: Meiling Yan; Xiaoyu Zhang; Qinxue Pu; Tao Huang; Qingdong Xie; Yan Wang; Jing Li; Yun Wang; Huan Gu; Tianhua Huang; Zhiling Li; Jiang Gu
• Keywords: Expression (computer science)

Abstract Immunoglobulin G (IgG), the major molecule of immune system, which was traditionally thought to be produced by differentiated B-lymphocytes, had recently been found in non-immune cells including spermatozoa rabbit testis. To study if human sperms could produce IgG that might play a role fertilization, we employed immunofluorescent staining, Western blot, situ hybridization, RT-PCR (reverse transcription polymerase chain reaction) and immunoelectron microscope were capable synthesizing IgG. protein mRNA detected cytoplasm, mainly neck region sperm immunoreactivity cover entire cell. The essential enzymes necessary for synthesis class switching, RAG1 (recombination activating gene 1), RAG2 2) AID (activation-induced cytidine deaminase), also cells. Furthermore, anti-IgG antibody inhibit from penetrating Zona-free hamster egg with statistical significance. These discoveries suggested immunoglobulin it during fertilization.