Differential neutrophil responses to bacterial stimuli: Streptococcal strains are potent inducers of heparin-binding protein and resistin-release

• DOI: 10.1038/srep21288
• Publication Date: 2016-02-18T12:02:44Z
• Authors: Johanna Snäll; Anna Linnér; Julia Uhlmann; Nikolai Siemens; Heike Ibold; Marton Janos; Adam Linder; Bernd Kreikemeyer; Heiko Herwald; Linda Johansson; Anna Norrby-Teglund
• Keywords: Resistin; Myeloma protein

Abstract Neutrophils are critical for the control of bacterial infections, but they may also contribute to disease pathology. Here we explore neutrophil responses, in particular release sepsis-associated factors heparin-binding protein (HBP) and resistin relation specific stimuli sepsis varying aetiology. Analyses HBP plasma septic patients revealed elevated levels as compared non-infected critically ill patients. correlated significantly patients, with strongest association seen group A streptococcal (GAS) cases. In vitro stimulation human neutrophils that fixed strains induced higher resistin, Staphylococcus aureus or Escherichia coli . Similarly, stimulated M1-protein showed a significant increase co-localization positive granules well exocytosis these factors, LPS. Using GAS strain deficient expression had negligible effect on activation, while stand-alone regulator MsmR was less stimulatory its wild type strain. Taken together, findings suggest activation is multifactorial involves, not limited to, proteins encoded by FCT-locus.