Microfluidic enrichment for the single cell analysis of circulating tumor cells

• DOI: 10.1038/srep22076
• Publication Date: 2016-02-29T12:07:16Z
• Authors: Trifanny Yeo; Swee Jin Tan; Chew Leng Lim; Dawn Ping Xi Lau; Yong Wei Chua; Sai Sakktee Krisna; Gopal Iyer; Gek San Tan; Tony Kiat Hon Lim; Daniel S.W. Tan; Wan-Teck Lim; Chwee Teck Lim
• Keywords: Circulating tumor cell; Single-Cell Analysis; T790M

Abstract Resistance to drug therapy is a major concern in cancer treatment. To probe clones resistant chemotherapy, the current approach conduct pooled cell analysis. However, this can yield false negative outcomes, especially when we are analyzing rare number of circulating tumor cells (CTCs) among an abundance other types. Here, develop microfluidic device that able perform high throughput, selective picking and isolation single CTC 100% purity from larger population cells. This effectively separate very CTCs blood samples as few 1 20,000 white We first demonstrate pure mixed track variations acquired T790M mutations before after treatment using model PC9 line. With clinical samples, then show isolated representative dominant EGFR such L858R found primary tumor. recovery device, potentially implement personalized not only through detecting genetic aberrations at level, but also tracking changes during anticancer therapy.