The interaction between Staphylococcus aureus SdrD and desmoglein 1 is important for adhesion to host cells

• DOI: 10.1038/srep22134
• Publication Date: 2016-02-29T12:53:22Z
• Authors: Fatemeh Askarian; Clement Ajayi; Anne-Merethe Hanssen; Nina M. van Sorge; Ingvild Pettersen; Dzung B. Diep; Johanna U. E. Sollid; Mona Johannessen
• Keywords: 0301 basic medicine; Staphylococcus aureus; 0303 health sciences; Desmoglein 1; Calcium-Binding Proteins; Ligands; Article; Bacterial Adhesion; Recombinant Proteins; Cell Line; 3. Good health; Lactococcus lactis; 03 medical and health sciences; HEK293 Cells; VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716; Bacterial Proteins; VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk mikrobiologi: 715; Genes, Bacterial; Host-Pathogen Interactions; Humans; VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711

AbstractStaphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors involved in colonization are adhesins such as the microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). Serine aspartate repeat containing protein D (SdrD) is involved in adhesion to human squamous cells isolated from the nose. Here, we identify Desmoglein 1 (Dsg1) as a novel interaction partner for SdrD. Genetic deletion of sdrD in S. aureus NCTC8325-4 through allelic replacement resulted in decreased bacterial adherence to Dsg1- expressing HaCaT cells in vitro. Complementary gain-of-function was demonstrated by heterologous expression of SdrD in Lactococcus lactis, which increased adherence to HaCaT cells. Also ectopic expression of Dsg1 in HEK293 cells resulted in increased adherence of S. aureus NCTC8325-4 in vitro. Increased adherence of NCTC8325-4, compared to NCTC8325-4ΔsdrD, to the recombinant immobilized Dsg1 demonstrated direct interaction between SdrD and Dsg1. Specificity of SdrD interaction with Dsg1 was further verified using flow cytometry and confirmed binding of recombinant SdrD to HaCaT cells expressing Dsg1 on their surface. These data demonstrate that Dsg1 is a host ligand for SdrD.