In vitro model of bone to facilitate measurement of adhesion forces and super-resolution imaging of osteoclasts

• DOI: 10.1038/srep22585
• Publication Date: 2016-03-03T11:04:15Z
• Authors: Takahiro Deguchi; Maria H. Alanne; Elnaz Fazeli; Katja M. Fagerlund; Paula Pennanen; Petri Lehenkari; Pekka E. Hänninen; Juha Peltonen; Tuomas Näreoja
• Keywords: Male; 0301 basic medicine; 03 medical and health sciences; Humans; Osteoclasts; Female; Microscopy, Interference; Bone Resorption; ta3111; Microscopy, Atomic Force; Models, Biological; Article

Abstract To elucidate processes in the osteoclastic bone resorption, visualise resorption and related actin reorganisation, a combination of imaging technologies an applicable vitro model is needed. Nanosized powder from matching species deposited on any biocompatible surface order to form thin, translucent, smooth elastic representation injured bone. Osteoclasts cultured layer expressed morphology ones sawed cortical slices. Resorption pits were easily identified by reflectance microscopy. The coating allowed structures interface be visualised with super-resolution microscopy along detailed interlinked networks branching conjunction V-ATPase, dynamin Arp2/3 at patches. Furthermore, we measured timescale adaptive osteoclast adhesion force spectroscopy experiments live osteoclasts bone-coated AFM cantilevers. Utilising advanced localised immunofluorescence signals respect high precision detected its early stages. Put together, our data supports cyclic for human osteoclasts.