Restoration of normal embryogenesis by mitochondrial supplementation in pig oocytes exhibiting mitochondrial DNA deficiency

• DOI: 10.1038/srep23229
• Publication Date: 2016-03-18T09:56:37Z
• Authors: Gael L. M. Cagnone; Te-Sha Tsai; Yogeshwar Makanji; Pamela Matthews; Jodee Gould; Michael S. Bonkowski; Kirstin D. Elgass; Ashley S. A. Wong; Lindsay E. Wu; Matthew McKenzie; David A. Sinclair; Justin C. St. John
• Keywords: 0301 basic medicine; 570; DNA Copy Number Variations; Swine; Gene Expression Profiling; Sus scrofa; Embryonic Development; Fertilization in Vitro; DNA, Mitochondrial; Article; Culture Media; Mitochondria; Embryo Culture Techniques; 03 medical and health sciences; Blastocyst; Pregnancy; Oocytes; Animals; Female

Abstract An increasing number of women fail to achieve pregnancy due either failed fertilization or embryo arrest during preimplantation development. This often results from decreased oocyte quality. Indeed, reduced mitochondrial DNA copy (mitochondrial deficiency) may disrupt quality in some women. To overcome deficiency, whilst maintaining genetic identity, we supplemented pig oocytes selected for cytoplasmic maturation and lower developmental competence, with autologous populations isolate at fertilization. Supplementation increased development blastocyst, the final stage development, promoted replication prior embryonic genome activation deficient but not normal levels DNA. Blastocysts exhibited transcriptome profiles more closely resembling those blastocysts developmentally competent oocytes. Furthermore, supplementation gene expression patterns associated metabolic disorders that were identified These demonstrate importance oocyte’s investment outcome subsequent