Dysregulated 14-3-3 Family in Peripheral Blood Leukocytes of Patients with Schizophrenia

• DOI: 10.1038/srep23791
• Publication Date: 2016-03-31T09:01:57Z
• Authors: Ying Qing; Liya Sun; Chao Yang; Jie Jiang; Xuhan Yang; Xiaowen Hu; Donghong Cui; Yifeng Xu; Lin He; Dongmei Han; Chunling Wan
• Keywords: Adult; Male; 0301 basic medicine; Severity of Illness Index; Article; 03 medical and health sciences; 14-3-3 Proteins; Gene Expression Regulation; Case-Control Studies; Leukocytes, Mononuclear; Schizophrenia; Humans; Protein Isoforms; Female; RNA, Messenger; Signal Transduction

Abstract The 14-3-3 family, which is composed of seven distinct members in humans, plays important roles the cell cycle, apoptosis, synaptic plasticity and neuronal differentiation migration. Previous genetic post-mortem gene expression studies have linked this family to schizophrenia. However, direction changes these has been inconsistent, reports living schizophrenic patients are still lacking. Here, we assessed protein levels peripheral blood leukocytes from drug-naïve first-episode matched controls. mRNA were quantified by qRT-PCR UPLC-MRM/MS, respectively. Expression analysis revealed four downregulated one upregulated transcripts as well five isoforms Moreover, significant positive correlations between found schizophrenia, also identified negative ε, θ ζ isoform symptoms Our results suggest that for dysregulated perhaps owing specific regulatory mechanisms, 14-3-3ε, genes could be useful indicators disease severity.