Overexpression of SlUPA-like induces cell enlargement, aberrant development and low stress tolerance through phytohormonal pathway in tomato

• DOI: 10.1038/srep23818
• Publication Date: 2016-03-30T09:16:45Z
• Authors: Baolu Cui; Zongli Hu; Jingtao Hu; Yanjie Zhang; Wencheng Yin; Zhiguo Zhu; Ye Feng; Guoping Chen
• Keywords: Genetically modified tomato

Abstract upa20 induces cell enlargement and hypertrophy development. In our research, overexpression of SlUPA-like , orthologous to severely affected the growth vegetative reproductive tissues. Wilted leaves curled upwardly sterile flowers were found in transgenic lines. Through anatomical analysis, palisade spongy tissues showed fluffy hypertrophic development plants. Gene expression analysis that GA responsive, biosynthetic signal transduction genes (e.g. GAST1 SlGA20OXs, SlGA3OXs SlGID1s SlPREs ) significantly upregulated, indicating response is stimulated by overproduction . Furthermore, was strongly induced exogenous JA wounding. Decreased PI-I SlJAZs (including SlJAZ2 SlJAZ10 SlJAZ11 observed plants, suggesting repressed. addition, overexpressed plant exhibited more opened stoma higher water loss than control when treated with dehydration stress, which related decreased ABA biosynthesis, response. Particularly, abnormal developments plants promote susceptibility Xanthomonas campestris pv. Therefore, it deduced from these results plays vital role regulation stress tolerance through GA, pathways.