Effects of Perfluorooctanoic Acid on Metabolic Profiles in Brain and Liver of Mouse Revealed by a High-throughput Targeted Metabolomics Approach

• DOI: 10.1038/srep23963
• Publication Date: 2016-04-01T09:08:53Z
• Authors: Nanyang Yu; Si Wei; Meiying Li; Jingping Yang; Kan Li; Ling Jin; Yuwei Xie; John P. Giesy; Xiaowei Zhang; Hongxia Yu
• Keywords: Perfluorooctanoic acid; Metabolome; Metabolic pathway; Neurotoxicity

Perfluorooctanoic acid (PFOA), a perfluoroalkyl acid, can result in hepatotoxicity and neurobehavioral effects animals. The metabolome, which serves as connection among transcriptome, proteome toxic effects, provides pathway-based insights into of PFOA. Since understanding changes the metabolic profile during neurotoxicity were still incomplete, high-throughput targeted metabolomics approach (278 metabolites) was used to investigate exposure PFOA for 28 d on brain liver male Balb/c mice. Results multivariate statistical analysis indicated that caused alterations pathways exposed individuals. Pathway suggested affected metabolism amino acids, lipids, carbohydrates energetics. Ten 18 metabolites identified potential unique biomarkers liver, respectively. In brain, concentrations neurotransmitters, including serotonin, dopamine, norepinephrine, glutamate novel mechanisms PFOA-induced effects. profiles lipids revealed involvement β-oxidation biosynthesis saturated unsaturated fatty acids hepatotoxicity, while arachidonic suggesting cause inflammation response liver. These results provide insight mechanism