MHC-class-II are expressed in a subpopulation of human neural stem cells in vitro in an IFNγ–independent fashion and during development

• DOI: 10.1038/srep24251
• Publication Date: 2016-04-15T09:18:10Z
• Authors: B. Vagaska; S. E. P. New; C. Alvarez-Gonzalez; F. D’Acquisto; S. G. Gomez; N. W. Bulstrode; A. Madrigal; P. Ferretti
• Keywords: Neurons; 0301 basic medicine; Histocompatibility Antigens Class I; Histocompatibility Antigens Class II; Gene Expression Regulation, Developmental; Cell Differentiation; Fetal Blood; Article; 3. Good health; Interferon-gamma; 03 medical and health sciences; Adipose Tissue; Neural Stem Cells; Astrocytes; Humans; Biomarkers; Receptors, Interferon

Abstract Expression of major histocompatibility antigens class-2 (MHC-II) under non-inflammatory conditions is not usually associated with the nervous system. Comparative analysis immunogenicity human embryonic/fetal brain-derived neural stem cells (hNSCs) and mesenchymal neurogenic potential from umbilical cord (UC-MSCs) paediatric adipose tissue (ADSCs), while highlighting differences in their immunogenicity, led us to discover subsets co-expressing marker SOX2 MHC-II antigen vivo during CNS development. proteins hNSCs are functional differently regulated upon differentiation along different lineages. Mimicking an inflammatory response using cytokine IFNγ induced up-regulation both astrocytes hNSCs, but UC-MSCs ADSCs, either undifferentiated or differentiated, though receptor expression was comparable. Together, hypoimmunogenicity ADSCs supports suitability for allogeneic therapy, significant progeny may at least part underlie negative effects reported some patients following embryonic cell grafts. Crucially, we show first time that developing brains restricted microglia as previously suggested, present discrete progenitors appears be independently stimuli.