CT-Finder: A Web Service for CRISPR Optimal Target Prediction and Visualization

• DOI: 10.1038/srep25516
• Publication Date: 2016-05-24T06:58:28Z
• Authors: Houxiang Zhu; Lauren Misel; Mitchell Graham; Michael L. Robinson; Chun Liang
• Keywords: Guide RNA; Genome Engineering

The CRISPR system holds much promise for successful genome engineering, but therapeutic, industrial, and research applications will place high demand on improving the specificity efficiency of this tool. CT-Finder (http://bioinfolab.miamioh.edu/ct-finder) is a web service to help users design guide RNAs (gRNAs) optimized specificity. accommodates original single-gRNA Cas9 two specificity-enhancing paired-gRNA systems: D10A nickases (Cas9n) dimeric RNA-guided FokI nucleases (RFNs). Optimal target candidates can be chosen based minimization predicted off-target effects. Graphical visualization on-target sites in provided validation. Major model organisms are covered by service.