Microfluidic platform combining droplets and magnetic tweezers: application to HER2 expression in cancer diagnosis
[SDV.IB] Life Sciences [q-bio]/Bioengineering
570
Receptor, ErbB-2
Magnetic Phenomena
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Microfluidics
610
[SDV.CAN]Life Sciences [q-bio]/Cancer
02 engineering and technology
01 natural sciences
Article
3. Good health
0104 chemical sciences
Calibration; Cell Line, Tumor; Humans; Microfluidics; Neoplasms; Receptor, ErbB-2; Magnetic Phenomena; Multidisciplinary
[SDV.CAN] Life Sciences [q-bio]/Cancer
Cell Line, Tumor
Neoplasms
Calibration
[SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology
Humans
[SDV.IB]Life Sciences [q-bio]/Bioengineering
0210 nano-technology
Molecular Biology
DOI:
10.1038/srep25540
Publication Date:
2016-05-09T09:25:29Z
AUTHORS (8)
ABSTRACT
AbstractThe development of precision medicine, together with the multiplication of targeted therapies and associated molecular biomarkers, call for major progress in genetic analysis methods, allowing increased multiplexing and the implementation of more complex decision trees, without cost increase or loss of robustness. We present a platform combining droplet microfluidics and magnetic tweezers, performing RNA purification, reverse transcription and amplification in a fully automated and programmable way, in droplets of 250nL directly sampled from a microtiter-plate. This platform decreases sample consumption about 100 fold as compared to current robotized platforms and it reduces human manipulations and contamination risk. The platform’s performance was first evaluated on cell lines, showing robust operation on RNA quantities corresponding to less than one cell and then clinically validated with a cohort of 21 breast cancer samples, for the determination of their HER2 expression status, in a blind comparison with an established routine clinical analysis.
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