Osthole inhibits histamine-dependent itch via modulating TRPV1 activity

• DOI: 10.1038/srep25657
• Publication Date: 2016-05-10T09:54:49Z
• Authors: Niu-Niu Yang; Hao Shi; Guang Yu; Chang-Ming Wang; Chan Zhu; Yan Yang; Xiao-Lin Yuan; Min Tang; Zhong-li Wang; Tana Gegen; Qian He; Kehua Tang; Lei Lan; Guan-Yi Wu; Zong-Xiang Tang
• Keywords: Capsaicin; Scratching; Antipruritic; Dorsal root ganglion

Abstract Osthole, an active coumarin isolated from Cnidium monnieri (L.) Cusson, has long been used in China as antipruritic herbal medicine; however, the antipruitic mechanism of osthole is unknown. We studied molecular histamine-dependent itch by behavioral test, Ca 2+ imaging and electrophysiological experiments. First, clearly remitted scratching behaviors mice induced with histamine, HTMT VUF8430. Second, cultured dorsal root ganglion (DRG) neurons, showed a dose-dependent inhibitory effect to histamine. On same also decreased response capsaicin In further tests, capsaicin-induced inward currents were inhibited osthole. These results revealed that modulating TRPV1 activity. This study will be helpful understanding how exerts anti-pruritus effects suggests may useful treatment medicine for itch.