Chiral amino acid metabolomics for novel biomarker screening in the prognosis of chronic kidney disease

• DOI: 10.1038/srep26137
• Publication Date: 2016-05-18T09:07:16Z
• Authors: Tomonori Kimura; Kenji Hamase; Yurika Miyoshi; Ryohei Yamamoto; Keiko Yasuda; Masashi Mita; Hiromi Rakugi; Terumasa Hayashi; Yoshitaka Isaka
• Keywords: Male; 0303 health sciences; Middle Aged; Prognosis; Article; 3. Good health; Plasma; 03 medical and health sciences; Isomerism; Humans; Metabolomics; Female; Amino Acids; Renal Insufficiency, Chronic; Biomarkers; Chromatography, High Pressure Liquid; Aged; Follow-Up Studies

Abstract D-Amino acids, the enantiomers of L-amino are increasingly recognized as novel biomarkers. Although amounts D-amino acids usually very trace in human, some them have sporadically been detected blood from patients with kidney diseases. This study examined whether multiple chiral amino would be associated functions, comorbidities, and prognosis chronic disease (CKD) by enantioselective analyses all a micro-two-dimensional high-performance liquid chromatograph (2D-HPLC)-based analytical platform. 16 out 21 were plasma 108 CKD longitudinal cohort. The levels D-Ser, D-Pro, D-Asn strongly function (estimated glomerular filtration ratio), D-Ala D-Pro age, level D-Asp presence diabetes mellitus. D-Ser significantly progression mutually-adjusted Cox regression analyses; risk composite end point (developing to ESKD or death before ESKD) was elevated 2.7- 3.8-fold those higher D-Asn. These findings identified potential biomarkers