Mucosal adherent bacterial dysbiosis in patients with colorectal adenomas

• DOI: 10.1038/srep26337
• Publication Date: 2016-05-20T20:15:53Z
• Authors: Yingying Lu; Jing Chen; Junyuan Zheng; Guoyong Hu; Jingjing Wang; Chunlan Huang; Lihong Lou; Xingpeng Wang; Yue Zeng
• Keywords: Dysbiosis; Colorectal adenoma

Abstract Recent reports have suggested that the gut microbiota is involved in progression of colorectal cancer (CRC). The composition CRC precursors has not been adequately described. To characterize structure adherent this disease, we conducted pyrosequencing-based analysis 16S rRNA genes to determine bacterial profile normal colons (healthy controls) and adenomas (CRC precursors). Adenoma mucosal biopsy samples adjacent colonic mucosa from 31 patients with 20 healthy volunteers were profiled using Illumina MiSeq platform. Principal coordinate (PCoA) showed structural segregation between adenomatous tissue control tissue. Alpha diversity estimations revealed higher adenomas. Taxonomic illustrated abundance eight phyla (Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Chloroflexi, Cyanobacteria, Candidate-division TM7 Tenericutes) was significantly different. In addition, Lactococcus Pseudomonas enriched preneoplastic tissue, whereas Enterococcus , Bacillus Solibacillus reduced. However, both PCoA cluster tree analyses similar non-adenoma tissues. These present findings provide preliminary experimental evidence supporting lesion may be most important factor leading alterations community composition.