The simple neuroendocrine-immune regulatory network in oyster Crassostrea gigas mediates complex functions

• DOI: 10.1038/srep26396
• Publication Date: 2016-05-20T20:31:20Z
• Authors: Zhaoqun Liu; Lingling Wang; Zhi Zhou; Ying Sun; Mengqiang Wang; Hao Wang; Zhanhui Hou; Dahai Gao; Qiang Gao; Linsheng Song
• Keywords: 0301 basic medicine; Neurotransmitter Agents; Protein Folding; 0303 health sciences; Hemocytes; MAP Kinase Signaling System; Sequence Analysis, RNA; Tumor Necrosis Factor-alpha; Gene Expression Profiling; NF-kappa B; Apoptosis; Article; 3. Good health; 03 medical and health sciences; Gene Expression Regulation; Heat Shock Transcription Factors; Phagocytosis; Animals; Crassostrea; Oxidation-Reduction

Abstract The neuroendocrine-immune (NEI) regulatory network is a complex system, which plays an indispensable role in the immunity of host. In present study, bioinformatical analysis transcriptomic data from oyster Crassostrea gigas and further biological validation revealed that TNF (CgTNF-1 CGI_10018786) could activate transcription factors NF-κB HSF (heat shock factor) through MAPK signaling pathway, then regulate apoptosis, redox reaction, neuro-regulation protein folding haemocytes. activated immune cells released neurotransmitters including acetylcholine, norepinephrine [Met 5 ]-enkephalin to response by arising expression three (CGI_10005109, CGI_10005110 CGI_10006440) translocating two (Cgp65, CGI_10018142 CgRel, CGI_10021567) between cytoplasm nuclei Neurotransmitters exhibited immunomodulation effects influencing apoptosis phagocytosis Acetylcholine down-regulate response, while up-regulate response. These results suggested simple might be virtue neurotransmitters, cytokines factors.