A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo

• DOI: 10.1038/srep26722
• Publication Date: 2016-05-24T17:18:35Z
• Authors: Ge Liu; Shan Kuang; Shimei Wu; Weihua Jin; Chaomin Sun
• Keywords: Viability assay; HeLa; MTT assay

Abstract Many polysaccharides isolated from plants have exhibited promising antitumor activities. The aim of this study is to investigate the activity novel polysaccharide named SPS Sargassum integerrimum , elucidate underlying anticancer mechanism in a human lung cancer cell line A549 and evaluate its anti-angiogenic both vitro vivo . results show that significantly reduces cells viability dose- time-dependent manner via MTT method. Flow cytometry analysis indicates could induce apoptosis, loss mitochondrial membrane potential (MMP), generation reactive oxygen species (ROS) G2/M phase cycle arrest cells. Up-regulation expressions P53 Bax, down-regulation expression Bcl-2 activation cleaved caspase-3, caspase-9 PARP are also detected by western blotting after treatment SPS. In addition, inhibits proliferation, migration cord formation umbilical vein endothelial (HUVECs) prevents vascular development zebrafish embryos Altogether, our data prove anti-angiogenesis properties provide further insights into pharmacological application as agent against cancer.