Annexin A1 translocates to nucleus and promotes the expression of pro-inflammatory cytokines in a PKC-dependent manner after OGD/R

• DOI: 10.1038/srep27028
• Publication Date: 2016-07-18T09:15:04Z
• Authors: Baoming Zhao; Jing Wang; Lu Liu; Xing Li; Shuangxi Liu; Qian Xia; Jing Shi
• Keywords: Annexin A1; Phorbol

Annexin A1 (ANXA1) is a protein known to have multiple roles in the regulation of inflammatory responses. In this study, we find that after oxygen glucose deprivation/reoxygenation (ODG/R) injury, activated PKC phosphorylated ANXA1 at serine 27 residue (p27S-ANXA1), and promoted translocation p27S-ANXA1 nucleus BV-2 microglial cells. This turn induced cells produce large amounts pro-inflammatory cytokines. The phenomenon could be mimicked by either transfecting mutant form with its converted aspartic acid, S27D, or using agonist, phorbol 12-myristate 13-acetate (PMA) these contrast, an S27A (serine alanine) treating antagonist, GF103209X (GF) reversed effet. Our study demonstrates can subsequently translocated OGD/R, resulting induction