miR-200 family promotes podocyte differentiation through repression of RSAD2

• DOI: 10.1038/srep27105
• Publication Date: 2016-06-02T09:16:29Z
• Authors: Zhigui Li; Hongqiang Yin; Shuang Hao; Lifeng Wang; Jing Gao; Xiaoyue Tan; Zhuo Yang
• Keywords: 0301 basic medicine; Binding Sites; Kidney Cortex; Base Sequence; Podocytes; Gene Expression; Proteins; Apoptosis; Cell Differentiation; Article; Up-Regulation; Mice; MicroRNAs; 03 medical and health sciences; Animals; RNA Interference; 3' Untranslated Regions; Cells, Cultured; Cell Proliferation

Abstract Mature podocytes are highly differentiated cells with several characteristic phenotypic features that involved in the glomerular filtration function. During kidney development, a series of changes morphological characteristics and cellular functions may happen podocytes. The miR-200 family various biological pathological processes. But underlying molecular mechanisms podocyte differentiation remain poorly understood. Herein is shown miR-200a, miR-200b miR-429 significantly upregulated during podocytes, highest upregulation miR-200a. In these cells, restraint by RNA interference assay revealed prominent inhibition cell differentiation. More intriguingly, directly inhibited radical S-adenosyl methionine domain-containing protein 2 (RASD2) expression. Moreover, further RSAD2 combining promotion dedifferentiation proliferation. addition, expression consistent vitro prenatal postnatal mouse down-regulated development. Together, findings indicate potentially promote through repression Our data also demonstrate novel role antiviral as regulator