MALDI-Imaging Mass Spectrometry: a step forward in the anatomopathological characterization of stenotic aortic valve tissue

• DOI: 10.1038/srep27106
• Publication Date: 2016-06-03T10:43:31Z
• Authors: Laura Mourino-Alv...; Ibon Iloro; Fernando de la Cu...; Mikel Azkargorta; Tamara Sastre-Oliva; Iraide Escobes; Luis F. Lopez-Alm...; Pedro L. Sanchez; Harkaitz Urreta; Francisco Fernand...; Angel Pinto; Luis R. Padial; Finn Akerström; Felix Elortza; Maria G. Barderas
• Keywords: Mass spectrometry imaging; MALDI imaging; Histology

Abstract Aortic stenosis (AS) is the most common form of valve disease. Once symptoms develop, there an inexorable deterioration with a poor prognosis; currently are no therapies capable modifying disease progression and aortic replacement only available treatment. Our goal to study calcification by matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS) get new insights at molecular level that could help in understanding this In work, we analyzed consecutive slices from tissue MALDI-IMS, establish spatial distribution proteins peptides directly surface histological sections. The analysis showed different structures corresponding regions observed conventional histology, including large areas zones rich collagen elastic fibers. Peptide extraction tissue, followed liquid chromatography analysis, provided identification VI α-3 NDRG2 which correlated masses obtained MALDI-IMS were confirmed immunohistochemistry. These results highlighted mechanism implied AS using novel technique never used before pathology. addition, can define specific proving complementary resolution histology.