Surfactant-activated lipase hybrid nanoflowers with enhanced enzymatic performance

• DOI: 10.1038/srep27928
• Publication Date: 2016-06-14T10:25:20Z
• Authors: Jiandong Cui; Yamin Zhao; Ronglin Liu; Cheng Zhong; Shiru Jia
• Keywords: Nanoflower; Thermostability

Abstract Increasing numbers of materials have been extensively used as platforms for enzyme immobilization to improve catalytic performance. However, activity the most enzymes was declined after immobilization. Here, we develop a surfactant-activated lipase-inorganic flowerlike hybrid nanomaterials with rational design based on interfacial activation and self-assembly. The resulting hybird nanoflower (activated hNF-lipase) exhibited 460% 200% higher than native lipase conventional (hNF-lipase). Furthermore, activated hNF-lipase displayed good reusability due its monodispersity mechanical properties, had excellent long-time stability. superior performances were attributed both conformational modulation surfactants hierarchical structure nanoflowers, which not only anchored lipases in an active form, but also decreased enzyme-support negative interaction mass-transfer limitations. This new biocatalytic system is promising find widespread use applications related biomedicine, biosensor, biodiesel.