Identification and characterization of a pathogenicity-related gene VdCYP1 from Verticillium dahliae

• DOI: 10.1038/srep27979
• Publication Date: 2016-06-23T05:30:47Z
• Authors: Dan-Dan Zhang; Xin-Yan Wang; Jie-Yin Chen; Zhi-Qiang Kong; Yue-Jing Gui; Nan-Yang Li; Yu-Ming Bao; Xiao-Feng Dai
• Keywords: Verticillium dahliae; Verticillium wilt; Secondary metabolism

Verticillium dahliae is a phytopathogenic fungus that causes vascular wilt disease in wide variety of crop plants, thereby causing extensive economic loss. In present study, one V. T-DNA mutant M01C06 showed the pathogenicity loss on cotton, and expression flanking gene encoding cytochrome P450 monooxygenase (P450, VdCYP1) was strongly repressed. P450s fungi could affect fungal by involving synthesis secondary metabolites. However, there no report about pathogenic function dahliae. VdCYP1 deletion complementation experiments confirmed pathogenicity-related A comparison culture supernatants mutants wild-type strains indicates at least 14 kinds metabolites syntheses were affected due to deletion. One these compounds, sulfacetamide, had ability induce necrosis wilting symptoms cotton. Above results indicate participate pathogenesis metabolism dahliae, such as compound sulfacetamide. conclusion, acts an important factor involve likely contributes process