Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity

• DOI: 10.1038/srep28763
• Publication Date: 2016-06-28T09:18:45Z
• Authors: Lavanya Moparthi; Tatjana I. Kichko; Mirjam Eberhardt; Edward D. Högestätt; Per Kjellbom; Urban Johanson; Peter W. Reeh; Andreas Leffler; Milos R. Filipovic; Peter M. Zygmunt
• Keywords: TRPM8

Abstract Thermosensitive Transient Receptor Potential (TRP) channels are believed to respond either cold or heat. In the case of TRP subtype A1 (TRPA1), there seems be a species-dependent divergence in temperature sensation as non-mammalian TRPA1 is heat-sensitive whereas mammalian sensitive cold. It has been speculated but never experimentally proven that and other temperature-sensitive ion have inherent capability responding both Here we show redox modification ligands affect human (hTRPA1) heat sensing properties lipid bilayer whole-cell patch-clamp recordings well heat-evoked TRPA1-dependent calcitonin gene-related peptide (CGRP) release from mouse trachea. Studies purified hTRPA1 intrinsic tryptophan fluorescence, absence bilayer, consolidate an bidirectional thermosensor modified by state ligands. Thus, property conserved mammalians, which may contribute warmth uncomfortable addition noxious