Identification and expression profiles of neuropeptides and their G protein-coupled receptors in the rice stem borer Chilo suppressalis

• DOI: 10.1038/srep28976
• Publication Date: 2016-06-29T11:16:23Z
• Authors: Gang Xu; Gui-Xiang Gu; Zi-Wen Teng; Shun-Fan Wu; Jia Huang; Qi-Sheng Song; Gong-Yin Ye; Qi Fang
• Keywords: Chilo suppressalis; Proctolin

Abstract In insects, neuropeptides play important roles in the regulation of multiple physiological processes by binding to their corresponding receptors, which are primarily G protein-coupled receptors (GPCRs). The genes encoding and associated GPCRs rice stem borer Chilo suppressalis were identified a transcriptomic analysis used identify potential targets for disruption protection crops. Forty-three candidate found encode neuropeptide precursors all known insect except arginine-vasopressin-like peptide (AVLP), CNMamide, neuropeptide-like 2-4 (NPLP2-4) proctolin. addition, novel alternative splicing variants three (allatostatin CC, CCHamide 1 short F) reported first time 51 putative identified. Phylogenetic analyses demonstrated that 44 these belong A-family (or rhodopsin-like), 5 B-family secretin-like) 2 leucine-rich repeat-containing GPCRs. These likely ligands also described. qRT-PCR revealed expression profiles GPCR various tissues C. . Our study provides fundamental information may further our understanding neuropeptidergic signaling systems Lepidoptera aid design peptidomimetics, pseudopeptides or small molecules capable disrupting regulated receptors.