Transcriptome sequencing and marker development in winged bean (Psophocarpus tetragonolobus; Leguminosae)

• DOI: 10.1038/srep29070
• Publication Date: 2016-06-30T10:08:57Z
• Authors: Mohammad Vatanparast; Prateek Shetty; Ratan Chopra; Jeff J. Doyle; N. Sathyanarayana; Ashley N. Egan
• Keywords: Expressed Sequence Tags; 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Gene Expression Regulation, Plant; Fabaceae; Trypsin Inhibitor, Kunitz Soybean; Transcriptome; Polymorphism, Single Nucleotide; Article; Microsatellite Repeats; Plant Diseases

Abstract Winged bean, Psophocarpus tetragonolobus (L.) DC., is similar to soybean in yield and nutritional value but more viable tropical conditions. Here, we strengthen genetic resources for this orphan crop by producing a de novo transcriptome assembly annotation of two Sri Lankan accessions (denoted herein as CPP34 [PI 491423] CPP37 639033]), developing simple sequence repeat (SSR) markers identifying single nucleotide polymorphisms (SNPs) between geographically separated genotypes. A combined based on 804,757 reads from produced 16,115 contigs with an N50 889 bp, over 90% which has significant similarity other legumes. Combining singletons 97,241 transcripts. We identified 12,956 SSRs, including 2,594 repeats primers were designed 5,190 high-confidence SNPs Nigerian The transcriptomic data sets generated here provide new gene discovery marker development will be vital future plant breeding efforts. also analyzed the trypsin inhibitor (STI) family, important defense genes, context related legumes found evidence radiation Kunitz (KTI) family within winged bean.