Biochemical and proteomic characterization of retrovirus Gag based microparticles carrying melanoma antigens

• DOI: 10.1038/srep29425
• Publication Date: 2016-07-12T12:56:39Z
• Authors: Reet Kurg; Olavi Reinsalu; Sergei Jagur; Kadri Õunap; Liisi Võsa; Sergo Kasvandik; Kärt Padari; Kiira Gildemann; Mart Ustav
• Keywords: Virus-like particle; Cancer Immunotherapy

Abstract Extracellular vesicles are membraneous particles released by a variety of cells into the extracellular microenvironment. Retroviruses utilize cellular vesiculation pathway for virus budding/assembly and retrovirus Gag protein induces spontaneous formation microvesicles or virus-like (VLPs) when expressed in mammalian cells. In this study, five different melanoma antigens, MAGEA4, MAGEA10, MART1, TRP1 MCAM, were incorporated VLPs their localization within was determined. Our data show that MAGEA4 MAGEA10 proteins as well MCAM on surface VLPs. The compartmentalization exogenously cancer antigens did not depend cell. Comparison content LC-MS/MS-based label-free quantitative proteomics showed carrying very similar to each other, but differ some extent from without recombinant antigen. We suggest based have potential be used immunotherapy.