M2 tumour-associated macrophages contribute to tumour progression via legumain remodelling the extracellular matrix in diffuse large B cell lymphoma

• DOI: 10.1038/srep30347
• Publication Date: 2016-07-28T12:11:11Z
• Authors: Long Shen; Honghao Li; Yuzhi Shi; Dekun Wang; Junbo Gong; Jing Xun; Sifan Zhou; Rong Xiang; Xiaoyue Tan
• Keywords: 0303 health sciences; Neovascularization, Pathologic; Macrophages; Gene Expression; Prognosis; Article; Collagen Type I; Extracellular Matrix; Fibronectins; Immunophenotyping; 3. Good health; Cysteine Endopeptidases; Disease Models, Animal; Mice; 03 medical and health sciences; Disease Progression; Animals; Heterografts; Humans; Lymphoma, Large B-Cell, Diffuse; Biomarkers

Abstract Effects of M2 tumour-associated macrophages on the pathogenesis diffuse large B cell lymphoma (DLBCL) are still controversial. Our data showed that number CD163-positive correlated negatively with DLBCL prognosis. Macrophage depletion by clodronate liposomes significantly suppressed tumour growth in a xenograft mouse model using OCI-Ly3 cells. Moreover, polarization induced legumain expression U937 Exogenous promoted degradation fibronectin and collagen I, which was abolished administration inhibitor RR-11a. Overexpression Raw 264.7 cells also tube formation endothelial matrigel. In DLBCL, decreased as well increased angiogenesis were found at late stage tumours compared early tumours. Co-localization observed extracellular matrix tissues. Administration to growth, deposition control. Taken together, our results suggest affect via overexpression therefore play an active role progression DLBCL.