Hydrogen prevents corneal endothelial damage in phacoemulsification cataract surgery

• DOI: 10.1038/srep31190
• Publication Date: 2016-08-08T09:14:00Z
• Authors: Tsutomu Igarashi; Ikuroh Ohsawa; Maika Kobayashi; Toru Igarashi; Hisaharu Suzuki; Masumi Iketani; Hiroshi Takahashi
• Keywords: Male; Phacoemulsification; Article; Cataract; Cornea; 03 medical and health sciences; 0302 clinical medicine; Animals; Endothelium; Rabbits; Oxidation-Reduction; Hydrogen

Abstract In phacoemulsification, ultrasound induces hydroxyl radical (·OH) formation, damaging corneal endothelium. Whether H 2 can prevent such oxidative damage in phacoemulsification was examined by vitro and vivo studies. dissolved a commercial irrigating solution. The effects of against ·OH generation were first confirmed electron-spin resonance (ESR) hydroxyphenyl fluorescein (HPF). ESR showed significantly decreased signal magnitude fluorescence intensity oxidized HPF less the -dissolved evaluated rabbits, comparing control solutions. Five hours after procedure, whole cornea excised subjected to image analysis for edema, real-time semiquantitative PCR (qPCR) heme oxygenase (HO)-1, catalase (CAT), superoxide dismutase 1 (SOD1) SOD2 mRNA immunohistochemistry. Corneal edema increases anti-oxidative HO-1, CAT expressions suppressed group. addition, endothelial cell two stress markers, 4-HNE 8-OHdG, lower conclusion, ocular solution protected cells from phacoemulsification-induced damage.