Mesp1 Marked Cardiac Progenitor Cells Repair Infarcted Mouse Hearts

• DOI: 10.1038/srep31457
• Publication Date: 2016-08-19T11:26:12Z
• Authors: Yu Liu; Li Chen; Andrea Diaz Diaz; Ashley Benham; Xueping Xu; Cori S. Wijaya; Faisal Fa’ak; Weijia Luo; Benjamin Soibam; Alon Azares; Wei Yu; Qiongying Lyu; M. David Stewart; Preethi Gunaratne; Austin Cooney; Bradley K. McConnell; Robert J. Schwartz
• Keywords: Progenitor

Abstract Mesp1 directs multipotential cardiovascular cell fates, even though it’s transiently induced prior to the appearance of cardiac progenitor program. Tracing Mesp1-expressing cells and their progeny allows isolation characterization earliest cells. Studying biology Mesp1-CPCs in culture ischemic disease models is an important initial step toward using them for heart treatment. Because Mesp1’s transitory nature, Mesp1-CPC lineages were traced by following EYFP expression murine Cre/+ ; Rosa26 EYFP/+ ES We captured EYFP+ that strongly expressed mesoderm markers transcription factors, but not pluripotent or nascent markers. BMP2/4 treatment led expansion cells, while Wnt3a Activin marginally effective. exposure readily endothelial smooth muscle inhibition canonical Wnt signaling was required enter cardiomyocyte fate. Injected mouse pre-contractile Mesp1-EYFP+ CPCs improved survivability injured mice restored functional performance infarcted hearts at least 3 months. are bona fide they integrated well emerged de novo into terminally differentiated myocytes, vascular