Bio- chemical and physical characterizations of mesenchymal stromal cells along the time course of directed differentiation

• DOI: 10.1038/srep31547
• Publication Date: 2016-08-16T10:04:40Z
• Authors: Yin-Quan Chen; Yi-Shiuan Liu; Yu-An Liu; Yi-Chang Wu; Juan C. del Álamo; Arthur Chiou; Oscar K. Lee
• Keywords: mechanobiology; Microrheology; Regenerative Medicine

Abstract Cellular biophysical properties are novel biomarkers of cell phenotypes which may reflect the status differentiating stem cells. Accurate characterizations cellular properties, in conjunction with corresponding biochemical could help to distinguish cells from primary cells, cancer and differentiated However, correlated evolution these course directed differentiation has not been well characterized. In this study, we applied video particle tracking microrheology (VPTM) measure intracellular viscoelasticity human mesenchymal stromal/stem (hMSCs). Our results showed that osteogenesis only increased both elastic viscous moduli, but also converted hMSCs viscous-like elastic-like. contrast, adipogenesis decreased moduli while remained during differentiation. bio- chemical physical parameters, such as gene expression profiles, morphology cytoskeleton arrangement, demonstrated VPTM is a unique approach quantify, high data throughput, maturation level anticipate their fate decisions. This suited for time-lapsed study mechanobiology especially three dimensional physico-chemical biomimetic environments including porous scaffolds.