3D-engineering of Cellularized Conduits for Peripheral Nerve Regeneration
0301 basic medicine
0303 health sciences
Stem Cells
Sciatic Nerve
Article
Nerve Regeneration
Rats
Rats, Sprague-Dawley
03 medical and health sciences
Adipose Tissue
Peripheral Nerve Injuries
Animals
Gelatin
Cryogels
DOI:
10.1038/srep32184
Publication Date:
2016-08-30T09:30:15Z
AUTHORS (13)
ABSTRACT
AbstractTissue engineered conduits have great promise for bridging peripheral nerve defects by providing physical guiding and biological cues. A flexible method for integrating support cells into a conduit with desired architectures is wanted. Here, a 3D-printing technology is adopted to prepare a bio-conduit with designer structures for peripheral nerve regeneration. This bio-conduit is consisted of a cryopolymerized gelatin methacryloyl (cryoGelMA) gel cellularized with adipose-derived stem cells (ASCs). By modeling using 3D-printed “lock and key” moulds, the cryoGelMA gel is structured into conduits with different geometries, such as the designed multichannel or bifurcating and the personalized structures. The cryoGelMA conduit is degradable and could be completely degraded in 2-4 months in vivo. The cryoGelMA scaffold supports the attachment, proliferation and survival of the seeded ASCs and up-regulates the expression of their neurotrophic factors mRNA in vitro. After implanted in a rat model, the bio-conduit is capable of supporting the re-innervation across a 10 mm sciatic nerve gap, with results close to that of the autografts in terms of functional and histological assessments. The study describes an indirect 3D-printing technology for fabricating cellularized designer conduits for peripheral nerve regeneration and could lead to the development of future nerve bio-conduits for clinical use.
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