3D-engineering of Cellularized Conduits for Peripheral Nerve Regeneration

• DOI: 10.1038/srep32184
• Publication Date: 2016-08-30T09:30:15Z
• Authors: Yu Hu; Yao Wu; Zhiyuan Gou; Jie Tao; Jiumeng Zhang; Qianqi Liu; Tianyi Kang; Shu Jiang; Siqing Huang; Jiankang He; Shaochen Chen; Yanan Du; Maling Gou
• Keywords: Electrical conduit; Nerve guidance conduit; Gelatin

Tissue engineered conduits have great promise for bridging peripheral nerve defects by providing physical guiding and biological cues. A flexible method integrating support cells into a conduit with desired architectures is wanted. Here, 3D-printing technology adopted to prepare bio-conduit designer structures regeneration. This consisted of cryopolymerized gelatin methacryloyl (cryoGelMA) gel cellularized adipose-derived stem (ASCs). By modeling using 3D-printed "lock key" moulds, the cryoGelMA structured different geometries, such as designed multichannel or bifurcating personalized structures. The degradable could be completely degraded in 2-4 months vivo. scaffold supports attachment, proliferation survival seeded ASCs, up-regulates expression their neurotrophic factors mRNA vitro. After implanted rat model, capable supporting re-innervation across 10 mm sciatic gap, results close that autografts terms functional histological assessments. study describes an indirect fabricating regeneration, lead development future bio-conduits clinical use.