A Multi-Serotype Approach Clarifies the Catabolite Control Protein A Regulon in the Major Human Pathogen Group A Streptococcus

• DOI: 10.1038/srep32442
• Publication Date: 2016-09-01T10:35:09Z
• Authors: Sruti DebRoy; Miguel Saldaña; Dante Travisany; Andrew Montano; Jessica Galloway-...; Nicola Horstmann; Hui Yao; Mauricio González; Alejandro Maass; Mauricio Latorre; Samuel A. Shelburne
• Keywords: CCPA; Regulon; Streptococcus Pyogenes

Catabolite control protein A (CcpA) is a highly conserved, master regulator of carbon source utilization in gram-positive bacteria, but the CcpA regulon remains ill-defined. In this study we aimed to clarify by determining impact CcpA-inactivation on virulence and transcriptome three distinct serotypes major human pathogen Group Streptococcus (GAS). significantly decreased GAS broad array animal challenge models consistent with idea that critical bacterial pathogenesis. Via comparative transcriptomics, established core enriched for conserved binding motifs (i.e. cre sites). Conversely, strain-specific differences seems consist primarily affected secondary networks. Refinement site composition via analysis facilitated development modified consensus shows promise improved prediction targets other medically relevant pathogens.