Improved Human Bone Marrow Mesenchymal Stem Cell Osteogenesis in 3D Bioprinted Tissue Scaffolds with Low Intensity Pulsed Ultrasound Stimulation

• DOI: 10.1038/srep32876
• Publication Date: 2016-09-06T10:23:36Z
• Authors: Xuan Zhou; Nathan J. Castro; Wei Zhu; Haitao Cui; Mitra Aliabouzar; Kausik Sarkar; Lijie Grace Zhang
• Keywords: Regenerative Medicine; 3D bioprinting

Abstract 3D printing and ultrasound techniques are showing great promise in the evolution of human musculoskeletal tissue repair regeneration medicine. The uniqueness present study was to combine low intensity pulsed (LIPUS) advanced synergistically improve growth osteogenic differentiation mesenchymal stem cells (MSC). Specifically, polyethylene glycol diacrylate bioinks containing cell adhesive Arginine-Glycine-Aspartic acid-Serene (RGDS) peptide and/or nanocrystalline hydroxyapatite (nHA) were used fabricate scaffolds with different geometric patterns via novel table-top stereolithography printer. resultant provide a highly porous interconnected environment support proliferation. Scaffolds small square pores determined be optimal pattern for MSC attachment growth. LIPUS working parameters 1.5 MHz, 20% duty cycle 150 mW/cm 2 intensity. Results demonstrated that RGDS nHA printed under treatment can greatly promote proliferation, alkaline phosphatase activity, calcium deposition total protein content. These results illustrate effectiveness combination biomimetic as valuable combinatorial tool improved function, thus make them promising future clinical various regenerative medicine application.