Genome-wide long non-coding RNA screening, identification and characterization in a model microorganism Chlamydomonas reinhardtii

• DOI: 10.1038/srep34109
• Publication Date: 2016-09-23T09:09:23Z
• Authors: Hui Li; Yuting Wang; Meirong Chen; Peng Xiao; Changxing Hu; Zhiyong Zeng; Chaogang Wang; Jiangxin Wang; Zhangli Hu
• Keywords: Chlamydomonas reinhardtii; Identification; Chlamydomonas

Abstract Microalgae are regarded as the most promising biofuel candidates and extensive metabolic engineering were conducted but very few improvements achieved. Long non-coding RNA (lncRNA) investigation manipulation may provide new insights for this issue. LncRNAs refer to transcripts that longer than 200 nucleotides, do not encode proteins play important roles in eukaryotic gene regulation. However, no information of potential lncRNAs has been reported alga. Recently, we performed sequencing Chlamydomonas reinhardtii , obtained totally 3,574 putative lncRNAs. 1440 considered high-confidence lncRNAs, including 936 large intergenic, 310 intronic 194 anti-sense The average transcript length, ORF length numbers exons much less genes green In contrast with human which more 98% spliced, percentage C. is only 48.1%. addition, identified 367 responsive sulfur deprivation, 36 photosynthesis-related This first time explored unicellular model organism . lncRNA data could also into hydrogen production under deprivation.