Measuring cell surface area and deformability of individual human red blood cells over blood storage using quantitative phase imaging

• DOI: 10.1038/srep34257
• Publication Date: 2016-10-04T14:56:39Z
• Authors: HyunJoo Park; SangYun Lee; Misuk Ji; Kyoohyun Kim; YongHak Son; Seongsoo Jang; YongKeun Park
• Keywords: Sphericity; Echinocyte; Erythrocyte deformability

Abstract The functionality and viability of stored human red blood cells (RBCs) is an important clinical issue in transfusions. To systematically investigate changes whole blood, the hematological properties individual RBCs were quantified samples for various periods with without a preservation solution called citrate phosphate dextrose adenine-1 (CPDA-1). With 3-D quantitative phase imaging techniques, optical measurements refractive index (RI) distributions membrane fluctuations done at cell level. From measurements, morphological (volume, surface area sphericity), biochemical (hemoglobin content concentration), mechanical parameters (dynamic fluctuation) simultaneously to functionalities progressive alterations RBCs. Our results show that CPDA-1 had dramatic transformation from discocytes spherocytes within two weeks which was accompanied by significant decreases deformability area, increases sphericity. However, maintained their morphology up 6 weeks.