M1/M2-macrophage phenotypes regulate renal calcium oxalate crystal development

• DOI: 10.1038/srep35167
• Publication Date: 2016-10-12T09:42:43Z
• Authors: Kazumi Taguchi; Atsushi Okada; Shuzo Hamamoto; Rei Unno; Yoshinobu Moritoki; Ryosuke Ando; Kentaro Mizuno; Keiichi Tozawa; Kenjiro Kohri; Takahiro Yasui
• Keywords: Osteopontin; Proinflammatory cytokine

Abstract In our previous report, M2-macrophage (Mφs) deficient mice showed increased renal calcium oxalate (CaOx) crystal formation; however, the role of Mφs-related-cytokines and chemokines that affect kidney stone formation remains unknown. Here, we investigated M1/M2s in development by using vitro vivo approaches. The phagocytic rate bone marrow-derived M2Mφs was higher than Mφs M1Mφs on co-culture with tubular cells (RTCs). However, amount attachment RTCs reduced M2Mφs. six hyperoxaluric C57BL/6J mice, M1Mφ transfusion induction LPS IFN-γ facilitated formation, whereas M2Mφ IL-4 IL-13 suppressed compared control. These treatments expression crystal-related genes, such as osteopontin CD44, treatment pro-inflammatory adhesion-related genes IL-6, inducible NOS, TNF-α, C3, VCAM-1. M2Mφ-related lower M1Mφ-related papillary tissue CaOx formers. Overall, results suggest is M1Mφs, but