Microfluidic co-culture system for cancer migratory analysis and anti-metastatic drugs screening

• DOI: 10.1038/srep35544
• Publication Date: 2016-10-20T09:43:32Z
• Authors: Shengli Mi; Zhichang Du; Yuanyuan Xu; Zhengjie Wu; Xiang Qian; Min Zhang; Wei Sun
• Keywords: 0303 health sciences; Breast Neoplasms; Microfluidic Analytical Techniques; Article; Coculture Techniques; 3. Good health; 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Lab-On-A-Chip Devices; Humans; Female; Drug Screening Assays, Antitumor; Neoplasm Metastasis

Abstract Tumour metastasis is an important reason for cancer death, and cell migration step in the process of tumour metastasis. Studying great significance. Here, we present a novel microfluidic co-culture system establish mild, moderate severe models by using HMEpiC MDA-MB–231 cells to study anti-cancer drug screening. Using this device, achieved high viability (over 90%) stable analysis ability cells. We observed that density determined probability occurrence metastatic induction normal affected velocity each cell. verified increase MDA-MB-231 co-cultured with was relative increased secretion IL-6 inhibitor assay. This also led decreased CK-14 morphological changes Finally, significant inhibition paclitaxel tamoxifen on observed. Taken together, our device could be useful tool quantitation migratory capability anti-metastatic