Establishment of a translational endothelial cell model using directed differentiation of induced pluripotent stem cells from Cynomolgus monkey

• DOI: 10.1038/srep35830
• Publication Date: 2016-10-25T09:44:06Z
• Authors: Eva C. Thoma; Tobias Heckel; David Keller; Nicolas Giroud; Brian Leonard; Klaus Christensen; Adrian Roth; Cristina Bertinet...; Martin Graf; Christoph Patsch
• Keywords: Vasculogenesis; Cell type; Directed differentiation; Cell Sorting

Abstract Due to their broad differentiation potential, pluripotent stem cells (PSCs) offer a promising approach for generating relevant cellular models various applications. While human PSC-based are already advanced, similar systems non-human primates (NHPs) still lacking. However, as NHPs the most appropriate animals evaluating safety of many novel pharmaceuticals, availability in vitro would be extremely useful bridge gap between and animal models. Here, we present NHP endothelial cell system using induced (IPSCs) from Cynomolgus monkey (Macaca fascicularis). Based on an adapted protocol IPSCs, directly differentiated macaque IPSCs into under chemically defined conditions. The resulting can enriched immuno-magnetic sorting display marker expression function. RNA sequencing revealed that process closely resembled vasculogenesis. Moreover, showed derived highly with respect gene patterns key functions, such inflammatory responses. These data demonstrate power IPSC technology generate types use translational compare type-specific responses across species.