Goblet Cell Hyperplasia Requires High Bicarbonate Transport To Support Mucin Release

• DOI: 10.1038/srep36016
• Publication Date: 2016-10-27T09:34:22Z
• Authors: Giulia Gorrieri; Paolo Scudieri; Emanuela Caci; Marco Schiavon; Valeria Tomati; Francesco Sirci; Francesco Napolitano; Diego Carrella; Ambra Gianotti; Ilaria Musante; Maria Favia; Valeria Casavola; Lorenzo Guerra; Federico Rea; Roberto Ravazzolo; Diego Di Bernardo; Luis J. V. Galietta
• Keywords: Bicarbonate

Abstract Goblet cell hyperplasia, a feature of asthma and other respiratory diseases, is driven by the Th-2 cytokines IL-4 IL-13. In human bronchial epithelial cells, we find that induces expression many genes coding for ion channels transporters, including TMEM16A, SLC26A4, SLC12A2, ATP12A. At functional level, enhances calcium- cAMP-activated chloride/bicarbonate secretion, resulting in high bicarbonate concentration alkaline pH fluid covering apical surface epithelia. Importantly, mucin release, elicited purinergic stimulation, requires presence basolateral solution defective cells derived from cystic fibrosis patients. conclusion, our results suggest induce profound change function multiple transporters enhanced transport ability. This required as an important mechanism to favor release clearance mucus.