Sex differences in colonization of gut microbiota from a man with short-term vegetarian and inulin-supplemented diet in germ-free mice

• DOI: 10.1038/srep36137
• Publication Date: 2016-10-31T10:51:19Z
• Authors: Jing-jing Wang; Jing Wang; Xiao-yan Pang; Li-ping Zhao; Ling Tian; Xing-peng Wang
• Keywords: UniFrac; Dysbiosis; Commensalism; Metabolome

Abstract Gnotobiotic mouse model is generally used to evaluate the efficacy of gut microbiota. Sex differences microbiota are acknowledged, yet effect recipient’s gender on bacterial colonization remains unclear. Here we inoculated male and female germ-free C57BL/6J mice with fecal bacteria from a man short-term vegetarian inulin-supplemented diet. We sequenced 16S rRNA genes V3-V4 region donor’s feces colonic content. Shannon diversity index showed recipients have higher than males. Weighted UniFrac principal coordinates analysis revealed overall structures were significantly separated those females, closer donor. Redundancy identified 46 operational taxonomic units (OTUs) differed between sexes. The relative abundance 13 OTUs in males, such as Parabacteroides distasonis Blautia faecis , while 33 overrepresented including Clostridium groups Escherichia fergusonii/Shigella sonnei . Moreover, interactions these differential sexually distinct. These findings demonstrated that intestine preferred accommodate differently. Therefore, it necessary designate gnotobiotic for complete evaluation modulatory effects human upon diseases.