Acrolein contributes strongly to antimicrobial and heterocyclic amine transformation activities of reuterin

0301 basic medicine 0303 health sciences Glyceraldehyde Biochemistry Article Anti-Bacterial Agents Gastrointestinal Microbiome 3. Good health Propane 03 medical and health sciences Humans Acrolein Biotechnology; Biochemistry Biotechnology
DOI: 10.1038/srep36246 Publication Date: 2016-11-07T10:08:57Z
ABSTRACT
AbstractGlycerol/diol dehydratases catalyze the conversion of glycerol to 3-hydroxypropionaldehyde (3-HPA), the basis of a multi-component system called reuterin. Reuterin has antimicrobial properties and undergoes chemical conjugation with dietary heterocyclic amines (HCAs). In aqueous solution reuterin is in dynamic equilibrium with the toxicant acrolein. It was the aim of this study to investigate the extent of acrolein formation at various physiological conditions and to determine its role in biological and chemical activities. The application of a combined novel analytical approach including IC-PAD, LC-MS and NMR together with specific acrolein scavengers suggested for the first time that acrolein, and not 3-HPA, is the active compound responsible for HCA conjugation and antimicrobial activity attributed to reuterin. As formation of the HCA conjugate was observed in vivo, our results imply that acrolein is formed in the human gut with implications on detoxification of HCAs. We propose to re-define the term reuterin to include acrolein.
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