Acrolein contributes strongly to antimicrobial and heterocyclic amine transformation activities of reuterin
0301 basic medicine
0303 health sciences
Glyceraldehyde
Biochemistry
Article
Anti-Bacterial Agents
Gastrointestinal Microbiome
3. Good health
Propane
03 medical and health sciences
Humans
Acrolein
Biotechnology; Biochemistry
Biotechnology
DOI:
10.1038/srep36246
Publication Date:
2016-11-07T10:08:57Z
AUTHORS (9)
ABSTRACT
AbstractGlycerol/diol dehydratases catalyze the conversion of glycerol to 3-hydroxypropionaldehyde (3-HPA), the basis of a multi-component system called reuterin. Reuterin has antimicrobial properties and undergoes chemical conjugation with dietary heterocyclic amines (HCAs). In aqueous solution reuterin is in dynamic equilibrium with the toxicant acrolein. It was the aim of this study to investigate the extent of acrolein formation at various physiological conditions and to determine its role in biological and chemical activities. The application of a combined novel analytical approach including IC-PAD, LC-MS and NMR together with specific acrolein scavengers suggested for the first time that acrolein, and not 3-HPA, is the active compound responsible for HCA conjugation and antimicrobial activity attributed to reuterin. As formation of the HCA conjugate was observed in vivo, our results imply that acrolein is formed in the human gut with implications on detoxification of HCAs. We propose to re-define the term reuterin to include acrolein.
SUPPLEMENTAL MATERIAL
Coming soon ....
REFERENCES (63)
CITATIONS (95)
EXTERNAL LINKS
PlumX Metrics
RECOMMENDATIONS
FAIR ASSESSMENT
Coming soon ....
JUPYTER LAB
Coming soon ....