Acrolein contributes strongly to antimicrobial and heterocyclic amine transformation activities of reuterin

• DOI: 10.1038/srep36246
• Publication Date: 2016-11-07T10:08:57Z
• Authors: Christina Engels; Clarissa Schwab; Jianbo Zhang; Marc J. A. Stevens; Corinne Bieri; Marc-Olivier Ebert; Kristopher McNeill; Shana J. Sturla; Christophe Lacroix
• Keywords: Acrolein; Conjugate; Chemical transformation; Toxicant

Abstract Glycerol/diol dehydratases catalyze the conversion of glycerol to 3-hydroxypropionaldehyde (3-HPA), basis a multi-component system called reuterin. Reuterin has antimicrobial properties and undergoes chemical conjugation with dietary heterocyclic amines (HCAs). In aqueous solution reuterin is in dynamic equilibrium toxicant acrolein. It was aim this study investigate extent acrolein formation at various physiological conditions determine its role biological activities. The application combined novel analytical approach including IC-PAD, LC-MS NMR together specific scavengers suggested for first time that acrolein, not 3-HPA, active compound responsible HCA activity attributed As conjugate observed vivo, our results imply formed human gut implications on detoxification HCAs. We propose re-define term include