The endotracheal tube microbiome associated with Pseudomonas aeruginosa or Staphylococcus epidermidis

• DOI: 10.1038/srep36507
• Publication Date: 2016-11-04T10:02:29Z
• Authors: An Hotterbeekx; Basil B. Xavier; Kenny Bielen; Christine Lammens; Pieter Moons; Tom Schepens; Margareta Ieven; Philippe G Jorens; Herman Goossens; Samir Kumar-Singh; Surbhi Malhotra-K...
• Keywords: Ventilator-associated Pneumonia

Abstract Ventilator-associated pneumonia (VAP) is one of the commonest hospital-acquired infections associated with high mortality. VAP pathogenesis closely linked to organisms colonizing endotracheal tube (ETT) such as Staphylococcus epidermidis and Pseudomonas aeruginosa , former a common commensal pathogenic potential latter known pathogen. However, recent gut microbiome studies show that pathogens rarely function alone. Hence, we determined ETT microbial consortium co-colonizing S. or P. understand its importance in development for patient prognosis. Using bacterial 16S rRNA fungal ITS-II sequencing on biomass showing presence and/or culture, found correlated inversely survival species diversity. A decision tree, using parameters, predict was generated. Patients relative abundance Pseudomonadaceae <4.6% Staphylococcaceae <70.8% had highest chance survival. When were >4.6%, age <66.5 years most important predictor These data indicate composition correlates prognosis, an outcome.