Enhancement of protein production via the strong DIT1 terminator and two RNA-binding proteins in Saccharomyces cerevisiae

• DOI: 10.1038/srep36997
• Publication Date: 2016-11-15T11:33:48Z
• Authors: Yoichiro Ito; Takao Kitagawa; Mamoru Yamanishi; Satoshi Katahira; Shingo Izawa; Kenji Irie; Makoto Furutani-S...; Takashi Matsuyama
• Keywords: Terminator (solar); Riboswitch; Coding region

Abstract Post-transcriptional upregulation is an effective way to increase the expression of transgenes and thus maximize yields target chemicals from metabolically engineered organisms. Refractory elements in 3′ untranslated region (UTR) that mRNA half-life might be available. In Saccharomyces cerevisiae , several terminator regions have shown activity increasing production proteins by upstream coding genes; among these terminators DIT1 has highest activity. Here, we found two resident trans -acting RNA-binding (Nab6p Pap1p) enhance through cis element GUUCG/U within 3′-UTR. These could upregulate a battery cell-wall–related genes. Mutagenesis improved its maximum 500% standard PGK1 terminator. Further understanding improvement this system will facilitate inexpensive stable complicated organism-derived drugs worldwide.